Composite

Part:BBa_J107206

Designed by: Lorenzo Pasotti   Group: Magni Lab   (2020-06-01)


HSL-inducible cassette for sgRNA targeting R0040

HSL-inducible device expressing an sgRNA specific for the PLtetO1 (BBa_R0040) promoter. A GFP expression cassette is also present to measure cell load.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2084
    Illegal BsaI site found at 2262
    Illegal BsaI.rc site found at 706
    Illegal BsaI.rc site found at 1839


[edit]
Categories
Parameters
None